Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 15;10:5192. doi: 10.1038/s41467-019-12913-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Electroporation of Dkk1 suppresses Lmx1a−/−;b−/− cortical gyrification. a An Lmx1a−/−;b−/− embryo in utero electroporated with Dkk1+ GFP at e12 and analyzed at e18. Early electroporation of Dkk1 into neocortical primordium restored lissencephaly (arrow), while the non-electroporated side remained gyrencephalic (arrowhead). b–h Lmx1a−/−;b−/− mutants electroporated with Dkk1+ GFP or GFP alone at e11.5 and analyzed at e15. Panels d, e are from sections adjacent to those shown in b, c and correspond to regions boxed in b, c. d–h In Dkk1-electroporated double mutants, the number of Pax6+ APs (arrows), Pax6+ /Tbr2− bRGs (open arrowheads) and Tbr2+ IPs (arrowheads) was reduced compared to GFP-electroporated double mutants (*p < 0.05, two-tailed t-test, n = 3 Dkk1-electroporated and n = 3 GFP-electroporated Lmx1a−/−;b−/− mutants). Bracket (d, e) indicates thickness of the Pax6+ VZ, which was also reduced in Dkk1-electroporated Lmx1a−/−;b−/−mutants. All data are mean ± s.d. Source data for panels f–h are provided as Source Data File. Scale bars: 400 μm (a); 200 μm (b, c); 100 μm (d, e)