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. 2019 Nov 15;10:5176. doi: 10.1038/s41467-019-13150-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — At E7.75, Tlr2-labeled progenitors contribute to embryonic and fetal hematopoiesis. a Experimental design of the fate mapping of cells with an active Tlr2 locus. b The percentage of EYFP⁺ embryos among all Tlr2^CreERT2Rosa26^EYFP embryos in the same litter pulsed with one dose of 4-OHT from E6.75 to E10.5 was analyzed at E17.5 by FCM (mean ± SEM; n = 3–8 litters). c Percentage of Tlr2^CreERT2EYFP⁺ cells in E17.5 fetal liver (FL, black dots) and E17.5 fetal thymus (FT, gray dots) in embryos described in (b); (mean ± SEM; n = 6–21 embryos). d The hematopoietic fate of Tlr2^CreERT2EYFP⁺ cells pulsed with one dose of 4-OHT from E7.75 to E10.5 was determined in E17.5 FL and FT by FCM; (mean ± SEM; n = 4–8). e Labeling of hematopoietic populations (including granulocytes and monocytes) was determined in E13.5 FL of Tlr2^CreERT2Rosa26^tdTomato embryos pulsed with a single dose of 4-OHT at E7.75 (mean ± SEM; n = 8; arp = average recombination probability assessed in Ter119^–c-kit^–CD45^– cells. f Gating strategy to identify the contribution of Tlr2^CreERT2EYFP⁺ cells to indicated hematopoietic populations in E11.5 YS and FL. g Fate tracing of E11.5 Tlr2^CreERT2EYFP⁺ cells pulsed with one dose of 4-OHT from E7.75 to E10.5 (mean ± SEM; n = 5–15) was analyzed by FCM according to (f). Source data are provided as a Source Data file