Fig. 5.

Tlr2-driven production of DTA efficiently deletes hematopoietic cells in E11.5 embryos. a Representative images of E11.5 Tlr2^wtRosa26^DTA and Tlr2^CreRosa26^DTA embryos (n = 4 independent experiments with 3–8 embryos of each genotype per experiment). FCM analysis (a) and its quantification (b) of TLR2⁺, c-kit⁺CD45^–, c-kit⁺CD45⁺, and c-kit^–CD45⁺ cell subsets in the YS, FL, caudal part, and head of dissected E11.5 Tlr2^wtRosa26^DTA (upper panel) and Tlr2^CreRosa26^DTA (lower panel) embryos (mean ± SEM; n = 4–5; ****p ≤ 0.0001, *p < 0.05; paired, two-tailed t test). Scale bar = 1 mm. Source data are provided as a Source Data file