Fig. 6.

E10.5 aortic pre-HSC activate their Tlr2 locus. a Cryosections of E10.5 (28–34sp) dorsal aortae with intra-aortic hematopoietic clusters (IAHCs) were analyzed from Tlr2^CreRosa26^EYFP embryos by staining for EYFP (green), c-kit (red) and CD31 (white). A representative image is shown (n = 5 independent experiments with 1–2 embryos per experiment). b Aortic regions were dissected from E10.5 Tlr2^CreRosa26^EYFP embryos (28–34sp), and hematopoietic populations as well as endothelial cells were analyzed for their labeling efficiency by FCM. The gating strategy for each indicated subset adopted from ref., ³⁸ is shown in Supplementary Fig 7a. c Sections of E10.5 (28–34sp) aortae were taken from Tlr2^CreRosa26^DTA (left panel) and Tlr2^wtRosa26^DTA (middle and right panels) embryos and stained for c-kit (green), CD31 (red), and CD45 (white) to identify IAHCs. Scale bar represents 50 µm. A representative image is shown (n = 5 independent experiments with 1–2 embryos of each genotype per experiment). d Aortic regions were disected from E10.5 Tlr2^wtRosa26^DTA (black dots) and Tlr2^CreRosa26^DTA (red dots) embryos (28–34sp). Hematopoietic populations as well as endothelial cells were analyzed for their frequencies by FCM (mean ± SEM; n = 4; *p < 0.05; paired, two-tailed t test). Gating strategy is shown in Supplementary Fig. 7b. Source data are provided as a Source Data file