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. 2019 Nov 15;9:16937. doi: 10.1038/s41598-019-53276-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aspirin enhances the effects of 5-Fu on suppressing tumor growth in vivo. (A) SW620 cells are injected subcutaneously into the left flanks of nude mice. 28 mice were randomized into four groups and treated with water, ASA alone (200 mg/kg, daily), FU alone (20 mg/kg, twice/week), and the combination of FU and ASA with the same dose and frequency, respectively. The xenografts are resected on day 23 and the tumor volumes were measured and analyzed. (B) The final body weight of mice in each group was shown. (C) SW620-FU cells were injected subcutaneously into the left flanks of 40 nude mice (Day 0). 40 mice were randomized into 8 groups and treated by the method described in the Materials and Methods and euthanized on day 23, and representative tumor images of each group were displayed. The tumor growth curve after treatment was analyzed and shown. (D) The representative photographs of tumors. (E) The curve of body weight growth was shown. The diagram presents the single measurements and the means ± SD (**P < 0.01, *P < 0.05).