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. Author manuscript; available in PMC: 2019 Nov 16.
Published in final edited form as: Cell Rep. 2019 Apr 23;27(4):1254–1264.e7. doi: 10.1016/j.celrep.2019.03.105

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Armenian Hamster Anti-Mouse CD3e ePluor® 450 Thermo Fisher Scientific Cat #48-0031-80; RRID: AB_10733280
Rat Anti-Human/Mouse CD45R (B220) eFluor® 450 Thermo Fisher Scientific Cat #48-0452-80; RRID: AB_1548763
Rat Anti-Mouse TER-119 eFluor® 450 Thermo Fisher Scientific Cat #48-5921-80; RRID: AB_1518809
Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) eFluor® 450 Thermo Fisher Scientific Cat #48-5931-82; RRID: AB_1548788
Rat Anti-Mouse/Human CD11b APC-Cy7 Biolegend Cat#101226; RRID: AB_830642
Rat Anti-Mouse Ly-6A/E (Sca-1) PE-Cy7 Biolegend Cat#108114; RRID: AB_493596
Rat Anti-Mouse CD117 (c-Kit) APC BD Biosciences Cat #553356; RRID: AB_398536
Armenian Hamster Anti-Mouse CD48 PITC Biolegend Cat#103404; RRID: AB_313019
Rat Anti-Mouse CD150 (SLAM) Brilliant Violet 510 Biolegend Cat#115929; RRID: AB_2562189
Rat Anti-Mouse CD16/CD32 (Mouse BD PC Block) BD Biosciences Cat #553142; RRID: AB_394657
Rat Anti-Mouse CD3 antibody PE-Cy7 Biolegend Cat#100220; RRID: AB_1732057
Rat Anti-Human/Mouse CD45R (B220) PITC Thermo Fisher Scientific Cat #11-0452-85; RRID: AB_465055
Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Biotin Biolegend Cat #108404; RRID: AB_313369
Mouse Anti-Mouse CD45.1 Pacific Blue Biolegend Cat #110722; RRID: AB_492866
Mouse Anti-Mouse CD45.2 APC Biolegend Cat#109814; RRID: AB_389211
Rat Anti-Mouse Lineage Cocktail Pacific Blue Biolegend Cat#133310; RRID: AB_11150779
Mouse Anti-Mouse CD45.2 Alexa Fluor® 700 Biolegend Cat #109822; RRID: AB_493731
Rat Anti-Mouse TER-119 APC Biolegend Cat#116212; RRID: AB_313713
Rat Anti-Mouse CD71 PITC BD Biosciences Cat #553266; RRID: AB_394743
Armenian Hamster Anti-Mouse CD3e Biotin Biolegend Cat#100304; RRID: AB_312669
Rat Anti-Human/Mouse CD45R (B220) Biotin Thermo Fisher Scientific Cat #13-0452-82; RRID: AB_466449
Rat Anti-Mouse CD19 Biotin Biolegend Cat #115504; RRID: AB_313639
Rat Anti-Mouse TER-119 Biotin Biolegend Cat#116204; RRID: AB_313705
Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Biotin Biolegend Cat #108404; RRID: AB_313369
eBioscience Streptavidin ePluor450 Thermo Fisher Scientific Cat #48-4317-82; RRID: AB_10359737
Rat Anti-Mouse CD34 PITC Thermo Fisher Scientific Cat #11-0341-85; RRID: AB_465022
Rat Anti-Mouse CD16/CD32 Alexa Fluor® 700 Thermo Fisher Scientific Cat #56-0161-82; RRID: AB_493994
Rat Anti-Mouse/Human CD11b APC Biolegend Cat#101212; RRID: AB_312795
Rat Anti-Mouse Ly-6G Pacific Blue Biolegend Cat#127612; RRID: AB_2251161
Rat Anti-Mouse Ly-6A/E (Sca-1) APC Biolegend Cat#108112; RRID: AB_313349
Rat Anti-Mouse CD45 APC-Cy7 BD Biosciences Cat #557659; RRID: AB_396774
Rat Anti-Mouse CD11b APC-Cy7 BD Biosciences Cat #557657; RRID: AB_396772
Rat Anti-Mouse Ter-119 APC-Cy7 Biolegend Cat#116223; RRID: AB_2137788
Rat Anti-Mouse Ly-6A/E (Sca-1) APC Thermo Fisher Scientific Cat #17-5981-82; RRID: AB_469487
Hamster Anti-Mouse/Rat CD29 (β1-integrin) PITC Biolegend Cat#102206; RRID: AB_312883
Rat Anti-Mouse CD184 (CXCR4) Biotin BD Biosciences Cat #551968; RRID: AB_394307
Anti-Myosin (Skeletal, Past) antibody Millipore Sigma Cat #M4276; RRID: AB_477190
Anti-Myosin (Skeletal, Slow) antibody Millipore Sigma Cat #M8421; RRID: AB_477248
Mouse Anti-Pax7 antibody Developmental Studies Hybridoma Bank Cat #AB_528428; RRID: AB_528428
Rabbit Anti-Laminin antibody Millipore Sigma Cat #AB2034; RRID: AB_91209
Goat Anti-mouse IgG1, Alexa Fluor 488 Thermo Fisher Scientific Cat #A-21121; RRID: AB_2535764
Goat Anti-rabbit IgG (H+L), Alexa Fluor 647 Thermo Fisher Scientific Cat #A-21244; RRID: AB_2535812
Mouse on Mouse (M.O.M.™) Basic Kit Vector Laboratories Cat #BMK-2202; RRID: AB_2336833
Streptavidin-PE-Cy7 BD Biosciences Cat #557598; RRID: AB_I0049577
CD45-ePluor450 Thermo Fisher Scientific Cat #48-0451-82; RRID: AB_1518806
CD140a (PDGPRa) -biotin Thermo Fisher Scientific Cat #13-1401-82; RRID: AB_466607
CD24-PITC Thermo Fisher Scientific Cat #11-0242-82; RRID: AB_464988
Sca-1-PerCP-Cy5.5 Thermo Fisher Scientific Cat #45-5981-82; RRID: AB_914372
Goat anti-mouse CD140a (PDGPRa) R&D systems Cat #AP1062-SP; RRID: AB_2236897
Rabbit anti-RPP Rockland Cat #600-401-379; RRID: AB_2209751
Alexa Fluor 488 Affinipure Donkey anti-goat IgG Jackson Immunoresearch Cat #705-545-147; RRID: AB_2336933
Cy ™ 3 Affinipure Donkey anti-rabbit IgG Jackson Immunoresearch Cat #711-165-152; RRID: AB_2307443
4’,6-diamino-2-phenyNndole (DAPI) Thermo Fisher Scientific Cat #D1306; RRID: AB_2629482
SYTOX Blue Dead Cell Stain, for flow cytometry Thermo Fisher Scientific Cat #S34857
7-AAD BD Biosciences Cat #559925
Streptavidin, Pacific Orange conjugate Thermo Fisher Scientific Cat #S32365
Anti-APC Microbeads Miltenyi Biotec Cat#120-001-265
Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific Cat#W11261
Propidium Iodide Millipore Sigma Cat#P4170-25MG
Calcein Blue, AM Thermo Fisher Scientific Cat#C1429
eBioscience™ Streptavidin-APC Thermo Fisher Scientific Cat#17-4317-82
Bacterial and Virus Strains
ElectroMAX™ Stbl4™ Competent Cells Thermo Fisher Scientific Cat#11635018
Chemicals, Peptides, and Recombinant Proteins
HBSS, calcium, magnesium, no phenol red Thermo Fisher Scientific Cat#14025-134
PBS (for cell culture) GE Healthcare (Hyclone) Cat#SH30071.03
PBS (for staining media) Millipore Sigma Cat#P6178-500mL
Dulbecco’s Phosphate-Buffered Saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat#14190-250
Collagen I Rat Protein Thermo Fisher Scientific Cat#A1048301
Natural Mouse Laminin, 1mg Thermo Fisher Scientific Cat#23017015
PGP-Basic, recombinant Millipore Sigma Cat #P0291
DMEM, high glucose Thermo Fisher Scientific Cat#11965118
Ham’s P10 Nutrient Mix, 500ml bottle Thermo Fisher Scientific Cat#11550043
Donor Horse Serum Atlanta Biologicals Cat#S12150
Penicillin-Streptomycin Thermo Fisher Scientific Cat#15140122
GlutaMAX Supplement Thermo Fisher Scientific Cat#35050061
Dexamethasone Millipore Sigma Cat#D1756
Insulin solution from bovine pancreas Millipore Sigma Cat#I0516
Rosiglitazone Cayman Chemical Cat#71740
3-isobutyl-1-methylxanthine Millipore Sigma Cat #I5879
Paraformaldehyde, 32% solution VWR Cat#100496-496
Triton X-100 Millipore Sigma Cat #T9284
Triton X-100 Thermo Fisher Scientific Cat#BP151
Normal Goat Serum, Jackson Immuno VWR Cat#102643-594
Donkey Serum Millipore Sigma Cat #D9663
Bovine Serum Albumin (BSA) Millipore Sigma Cat #A9647
Bovine Serum Albumin (BSA) Millipore Sigma Cat #A7030
Gelatin from cold water fish skin Millipore Sigma Cat #G7765
Tween 20 Millipore Sigma Cat #P1379
BODIPY™ 493/503 Thermo Fisher Scientific Cat #D3922
Hoechst 33342, Trihydrochloride, Trihydrate Thermo Fisher Scientific Cat #H1399
Cardiotoxin gamma from Naja pallida Latoxin Cat #L8102
EDTA (0.5M), pH 8.0 Thermo Fisher Scientific Cat #AM9261
Dextran Millipore Sigma Cat #31392
ACK Lysing Buffer Thermo Fisher Scientific Cat #A1049201
Iodixanol Axis-Shield PoC AS Cat #AXS-1114542-5
Trizol LS Thermo Fisher Scientific Cat #1029610
Isopentane (2-Methylbutane) Millipore Sigma Cat #M32631
Paraformaldehyde (PPA), 32% solution, EM grade VWR Cat #100496-496
Vectashield HardSet Antifade Mounting Medium with DAPI Vector Laboratories Cat #H-1200
Critical Commercial Assays
SuperScript IV VILO Master Mix with ezDNase Enzyme Thermo Fisher Scientific Cat #11766050
Pax7 TaqMan assay Thermo Fisher Scientific Mm01354484_m1
Myf5 TaqMan assay Thermo Fisher Scientific Mm00435125_m1
Myod1 TaqMan assay Thermo Fisher Scientific Mm00440387_m1
Myog TaqMan assay Thermo Fisher Scientific Mm00446194_m1
Gapdh TaqMan assay Thermo Fisher Scientific Mm99999915_g1
TaqMan Past Advanced Master Mix Thermo Fisher Scientific Cat #4444557
Experimental Models: Cell Lines
HEK293 ATCC Cat #CRL-1573; RRID: CVCL_0045
Experimental Models: Organisms/Strains
Mouse: C57B/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801; RRID: IMSR_JAX:001801
Mouse: B6;129S6-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory JAX: 007905; RRID: IMSR_JAX:007905
Mouse: B6.Cg-Tg(Pax7-ZsGreen)1Kyba/J The Jackson Laboratory JAX: 029549; RRID: IMSR_JAX:029549
Mouse: B6.SJL-Ptprca Pepcb/BoyJ The Jackson Laboratory JAX: 002014; RRID: IMSR_JAX:002014
Mouse: C57BL/6J The Jackson Laboratory JAX: 000664; RRID: IMSR_JAX:000664
Oligonucleotides
Pax7_zsGreen_F; CTGCATGTACCACGAGTCCA N/A
Pax7_zsGreen_R; GTCAGGTGCCACTTCTGGTT N/A
tdTomato_WT_F; AAGGGAGCTGCAGTGGAGTA N/A
tdTomato_WT_R; CCGAAAATCTGTGGGAAGTC N/A
tdTomato_Knock-in_F; CTGTTCCTGTACGGCATGG N/A
tdTomato_Knock-in_R; GGCATTAAAGCAGCGTATCC N/A
Recombinant DNA
Package plasmid (pAAV2/Anc80) L65AAP) Addgene Cat #92307
Transgene plasmid (pAAV Cre) GTVC In house production
pAAV-Cre control plasmid Cell Biolabs AAV-401
Helper plasmid (ΔP6) GTVC In house production
Package plasmid (pKAAV2/8, pKAAV2/9) GTVC In house production
REAGENT or RESOURCE SOURCE IDENTIFIER
Software and Algorithms
BD PACSDiva™ Software v8.0.2 BD Biosciences http://www.bdbiosciences.com/us/instruments/research/software/flow-cytometry-acquisition/bd-facsdiva-software/m/111112/overview ;
RRID: SCR_001456
FlowJo v10.1r5 FlowJo https://www.flowjo.com/solutions/flowjo;
RRID: SCR_008520
Other
Covidien Monoject Softpack Insulin Syringe, 1/2mL, 28Gx 1/2” Covidien 1188528012
Equisul-SDT® (Sulfadiazine Trimethoprim) Aurora Pharmaceutical Cat #28002
Irradiated PicoLab Mouse Diet 20 5058 LabDiet, St. Louis, MO Cat #0007689
Irradiated LabDiet Prolab Isopro RMH 3000 5P75 LabDiet, St. Louis, MO Cat #0006972
Tissue-Tek® O.C.T. Compound, Sakura® Pinetek VWR 25608-930