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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Gut. 2019 May 16;69(2):264–273. doi: 10.1136/gutjnl-2018-316830

Figure 2.

Figure 2

IL23R-induced Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathways are required for IL23-induced cytokines in monocyte-derived macrophages (MDMs). (A, C) MDMs (n=6 for each) were treated with 10 ng/mL IL23 for 1 hour. Representative flow cytometry with mean fluorescence intensity (MFI) values and summary graphs with fold increase MFI+SEM. Isotype control shown for treated cells. (B, D) MDMs (n=6 for each) were transfected with scrambled or the indicated (B) JAK member or (D) STAT member siRNA. Cells were then treated with 10 ng/ mL IL23 for 24 hours. Mean cytokine secretion+SEM. Significance is compared with scrambled siRNA-transfected, IL23-treated cells. Similar results were seen in an independent n=6 for (C) and (D), and n=4 for (B). *P<0.05; **P<0.01; ***P<0.001; †P<1×10−4; ††P<1×10−5. IL, interleukin; NS, not significant; NT, no treatment; scr, scrambled; TNF, tumour necrosis factor; Tx, treatment.