. Author manuscript; available in PMC: 2020 Nov 2.

Published in final edited form as: J Physiol. 2019 Nov 2;597(22):5495–5514. doi: 10.1113/JP278168

Table 1.

Time course of glutamate-activated NMDA receptor responses.

GluN1–1a	rise time (ms)	τ_fast (ms)	τ_slow (ms)	% fast	τ_w (ms)	n
A/A	3.9 ± 0.3	87 ± 7	-	100^a	87 ± 7^#,$	7
A_C1/A_C2	4.2 ± 0.9	78 ± 13	-	100^a	78 ± 13^*^,#,$	7
A_C1/B_C2	10.9 ± 6.2	93 ± 27	180 ± 62	63 ± 19	140 ± 10^*^,#,$	5
B/B	14.9 ± 1.2	240 ± 23	1020 ± 72	59 ± 5	548 ± 45^*^,$	12
B_C1/B_C2	15.1 ± 2.7	205 ± 35	1045 ± 129	58 ± 8	533 ± 58^*^,$	8
B_C1/D_C2	10.9 ± 2.5	516 ± 42	3521 ± 446	54 ± 6	1767 ± 117^*^,#,$	6
D/D	6.2 ± 0.7	832 ± 146	7436 ± 890	24 ± 3	5714 ± 458^*^,#	7
D_C1/D_C2	8.6 ± 4.8	527 ± 225	6912 ± 848	17 ± 7	5700 ± 561^*^,#	4

GluN1–1b	rise time (ms)	τ_fast (ms)	τ_slow (ms)	% fast	τ_w (ms)	n
A/A	4.8 ± 0.3	56 ± 6	-	100^a	56 ± 6^#,$	6
A_C1/A_C2	6.3 ± 2.8	53 ± 7	-	100^a	53 ± 7^#,$	5
A_C1/B_C2	3.7 ± 0.5	47 ± 4	160 ± 25	69 ± 12	74 ± 5^*^,#,$	5
B/B	10.3 ± 1.5	81 ± 8	208 ± 49	58 ± 12	155 ± 19^*^,$	6
B_C1/B_C2	8.3 ± 1.6	101 ± 15	360 ± 89	84 ± 6	138 ± 13^*^,$	7
B_C1/D_C2	9.2 ± 1.3	127 ± 31	634 ± 132	50 ± 15	288 ± 22^*^,#,$	8
D/D	3.5 ± 0.4	245 ± 32	1609 ± 148	40 ± 7	992 ± 68^*^,#	10
D_C1/D_C2	4.5 ± 0.7	256 ± 92	1399 ± 141	14 ± 4	1230 ± 86^*^,#	5

Responses from NMDA receptors expressed in Xenopus oocytes were measured using outside-out patch-clamp recordings. Naming of subtypes is such that e.g. A/A indicates diheteromeric GluN1/2A/2A receptors and A_C1/A_C2 indicates diheteromeric GluN1/2A_C1/2A_C2 receptors that contain the C1 and the C2 tags. Responses were activated by a brief application (3–5 ms) of 1 mM glutamate in the continuous presence of 100 μM glycine. Rise times are for 10–90% of the response amplitude, deactivation time constants were determined using two-exponential fits to obtain τ_fast, τ_slow, and % fast is the fitted percentage of the fast component.

indicates that only a single-exponential was fitted to the deactivation time course. Weighted time constants (τ_w) were calculated as τ_w = τ_fast · I_fast/(I_fast + I_slow) + τ_slow · I_slow/(I_fast + I_slow). Data are mean ± SEM, and n is the number of patches used to generate the data. Deactivation rates (i.e. 1/τ_w) were used for statistical comparisons using one-way ANOVA with Tukey’s post hoc test.

indicates significantly different from A/A,

indicates significantly different from B/B, and ^§indicates significantly different from D/D.