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ATDC5 cell morphology on GF. Transmitted light and fluorescence microscopy of ATDC5 cells grown on bare three-dimensional GF for 28 days. (A and E) GF imaged by transmitted light microscopy, (B and F) Blue, nuclei (DAPI); (C and G) Green, F-actin (Alexa Fluor 488 phalloidin); (D and H) Overlay of transmitted light, DAPI, and phalloidin staining. (A–D) Scale-bar: 50 μm. (E–H) Scale-bar: 20 μm.
