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. Author manuscript; available in PMC: 2020 Mar 30.
Published in final edited form as: Nat Struct Mol Biol. 2019 Sep 30;26(10):919–929. doi: 10.1038/s41594-019-0297-8

Fig. 2. Cryo-EM structure of the SecA•RNCRodZ.

Fig. 2

a, Overview of the cryo-EM model of the SecA•RNCRodZ complex. Ribosomal RNA is in grey, ribosomal proteins in the large and small subunits are in blue and yellow, respectively, the P-site tRNA is in beige, and EM-densities of the ribosome and SecA are from the global refinement and focused refinement, respectively, and are filtered to the respective resolutions of 3.1 and 5.7 Å. b, Representative fit of the RodZ TMD and the contact points between the ribosome and SecA with the overlaid EM-density (grey mesh). The ribosomal protein uL23 and the RodZ TMD are colored in green and magenta, respectively. c, Representative fit of the SecA model with the overlaid EM-density obtained from the focused refinement approach.