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. 2019 Nov 15;2(6):e201900422. doi: 10.26508/lsa.201900422

Figure 5. LecB localises in LC3- and RAB9-positive compartments.

Figure 5.

(A, B) Confocal micrographs with respective colocalisation quantification of keratinocytes stimulated with 5 μg/ml of fluorescently labelled LecB (green). Panels indicated as “+LecB” refer to 12 h incubation. (A, B) After fixation and permeabilisation, the cells were stained for LC3 (A) and RAB9 (B) (both in red). Graphs report the mean value ± SEM of Mander’s overlap coefficients calculated from at least three independent experiments. **** denotes P < 0.0001; one-way ANOVA was used for statistical analysis. Scale bar: 10 μm. (C, D) Western blot of eluted samples from time series pull-down assays using biotinylated LecB (5 μg/ml). (C, D) Membranes were probed for LecB, IGF-1R (C) and ubiquitin (D).