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. 2019 Nov 15;50:95. doi: 10.1186/s13567-019-0714-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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wbdR tagged vaccines can be identified by a modified multiplex PCR assay. Identification and differentiation of Brucella species, classical vaccines and Rev1 wbdR tagged vaccines by a modified Bruce-Ladder [31] that includes a new pair of primers based on the wbdR gene that amplify a band of 347 bp specific for tagged vaccines. C–(H₂O) and PM (1 Kb plus DNA ladder) used as molecular size marker.