Fig. 6.

Lack of PIM1 target sites reduces also the ability of NFATC1 to enhance invasiveness of prostate cancer cells. a For invasion assays, PC-3 cells were grown in Boyden chambers in the absence (−) or presence (+) of 10 μM DHPCC-9. After 48 h, cells that had invaded through the membranes were fixed, stained with crystal violet and counted. Shown are relative invasion rates from two separate experiments with triplicate samples, the results of which had been normalized against the mock-transfected control samples. Shown are also representative pictures of the effects of wild-type (WT) or multi mutant (MM) NFATC1 on cell invasion after 48 h. Scale bar 500 μm. b Matrix metalloprotease (MMP) expression levels were measured by gelatinase activity assays from invasion sample media. Shown are relative MMP-2/MMP-9 expression levels from two separate experiments with three parallel samples