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. 2019 Nov 15;19:313. doi: 10.1186/s12906-019-2704-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effects of JPYW treatment on the expression of aging-related genes. The expression levels of aging-related genes were determined by qRT-PCR using the 2^−ΔΔCT method in worms with or without 150 μg/ml JPYW treatment at 20 °C. The graph shows the mean and SEM values from two independent experiments. Compared to the control treatment, JPYW treatment significantly changed the expression levels of the genes daf-16, clk-2, skn-1 and sir-2.1 (P < 0.05), but not those of the gene sod-3 (P > 0.05)