Table 4.

Summary of the species identification, nucleotide differences range and amount of available reference sequences based on 16S rRNA, sodA, tuf and rpoB genes and 16S–23S rRNA region for Streptococcus genus

	16S rRNA gene	sodA gene	tuf gene	rpoB gene	NGS 16S–23S rRNA
Unambiguous species identification	25 species (60%)	34 species (81%)	39 species (93%)	39 species (93%)	39 species (93%)
The lowest amount of nucleotides differences	0	0	0	0	2
The highest amount of nucleotides differences	228	176	205	186	1209
No. of species without reference sequences in the databases	0	8	9	5	15