Fig. 6.

Activation of pyroptosis in fetal mice and offspring of diabetic dams. mRNA (a) and protein (b) levels of NOD-like receptor family pyrin domain containing 3 (NLRP3) and apoptosis-associated speck-like protein (Asc) in macrophages isolated from brain tissue of 18.5-day-old fetal mice (five to eight fetal brains). c Protein levels of pro-caspase-1, pro-interleukin (IL)-1β, and pro-IL-18 in macrophages (left) and levels of caspase-1, IL-1β, and IL-18 in the culture supernatants of macrophages (right). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the internal control. d Active caspase-1-positive cells in macrophages isolated from the brain tissues of 18.5-day-old fetal mice. The levels of IL-1βand IL-18 (e) in the brains of 18.5-day-old fetal mice and 7-day-old offspring (pools of three whole brains). *Chemerin-induced diabetic dams vs. controls (E18.5 days), #chemerin-induced diabetic dams with ChemR23 knockdown vs. chemerin-induced diabetic dams (E18.5 days), ^chemerin-induced diabetic dams vs. controls (B7.0 days), and $chemerin-induced diabetic dams with ChemR23 knockdown vs. chemerin-induced diabetic dams (B7.0 days). * and #—P < 0.05; **, ##, ^^, and $$—P < 0.01. Scale bar: 50 μm