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. 2019 Nov 15;10:323. doi: 10.1186/s13287-019-1413-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miR-326-5p promoted EPC angiogenesis in vitro and in vivo. a Tube formation assay on Matrigel was assessed 6 h after seeding HUVECs pretreated with DiI-labeled EPCs/NC, miR-326-5p agomir EPCs, miR-326-5p antagomir EPCs, or miR-326-5p agomir EPCs+ Wnt1 agonist. DiI-positive incorporated EPCs were observed in the HUVEC-formed tube structure (red). Bar, 200 μm. b The number of DiI-positive incorporated EPCs was counted and averaged in each group (n = 4). c Gross look of Matrigel plugs. Bar, 5 mm. d Capillaries of immunofluorescence were observed (red, bar 50 μm) in the sections of Matrigel plugs and e were counted and averaged in each group (n = 5). The P values for comparison were indicated in the images, respectively