Table 1.
PCR Primers Used For Construction In This Study
| Primers | Sequence(5ʹ–3ʹ) | Size Of Product (bp) | Positions In 26,695 DNA Sequence(No.NC_000915.1) |
|---|---|---|---|
| F1 | CTATAGGGCGAATTGGGTACCCCCATTCAAGCCAATTTTTTCC | 718 | From 1,261,390 to 1,262,065 |
| R1 | TAAAACCGCCCAGTCTCGAG ACGCATGCGTTTGGGTGC | ||
| F2 | TCTCGAGGCCGCTCACACTCTTTTAGAAGA | 777 | From 1,263,056 to 1,263,804 |
| R2 | AAAGCTGGAGCTCCACCGCGGTTTTAAAGGGCCTAAATGACGC | ||
| F3 | ATGCGTCTCGAGACTGGGCGGTTTTATGGACA | 1214 | |
| R3 | CTAAAAGAGTGTGAGCGGCCTCGAGAAATTAAAAATGAAGTTTTAGCACG TG | ||
| F4 | AAGGGCTATTGATGAGCGTA | 1066 or 1199 | From 1,262,025 to 1,263,090 |
| R4 | GGCGTTTAAGGCTCTTCT | ||
| F5 | TCTCGAGTGCGGGAAAACAAGCATTG | 537 | From 1,262,771 to 1,263,279 |
| R5 | AAAGCTGGAGCTCCACCGCGGACTCAGTGGCCTAATGGCTTTGACT | ||
| F6 | ATGCGTCTCGAGACTGGGCGGTTTTATGGACA | 1214 | |
| R6 | CAATGCTTGTTTTCCCGCACTCGAGAAATTAAAAATGAAGTTTTAGCACG TG | ||
| F7 | AAGGGCTATTGATGAGCGTA | 1068 or 2257 | From 1,262,025 to 1,263,092 |
| R7 | AAGGCGTTTAAGGCTCTTC | ||
| F8 | CTATAGGGCGAATTGGGTACCTTGAGTGTTGAGAATTTAGAAAAATGG | 534 | From 174,691 to 175,187 |
| R8 | GTTTCAGTCGCTCGAGTCACTCTGTGTTTTCTCGCTCCA | ||
| F9 | CACAGAGTGACTCGAGCGACTGAAACTTTCGCAAGCA | 1228 | From 1,261,884 to 1,263,085 |
| R9 | GTGCCGATCATTAAGGCTCTTCTAAAAGAGTGTGAGC | ||
| F10 | GACGATATGATCATCTCGAGCCATGAAAACAAAGCCTTAAATTTC | 813 | From 173,857 to 174,631 |
| R10 | AAAGCTGGAGCTCCACCGCGGCGCAAACGGCCAATGATC | ||
| F11 | AGAGCCTTAATGATCGGCACGTAAGAGGTTC | 1050 | |
| R11 | TCATGGCTCGAGATGATCATATCGTCAATTATTACCTCCA | ||
| F12 | TGGAATCTTTGGAGCGAG | 594 or 2852 | From 174,112 to 174,705 |
| R12 | AGCGATTGGATGCGAGCTA | ||
| F13 | CTCAACACTTTAGGGGTAATCAT | 1347 | From 1,261,953 to 1,263,299 |
| R13 | AAACCTAAAAACTCAGTGGTCTAA |
Note: Underlined part indicates overlapping DNA sequences.