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. 2019 Nov 12;11:9585–9597. doi: 10.2147/CMAR.S215631

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© 2019 Xu et al.

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CCDC132 is essential for GC cell proliferation and survival. (A) and (C) the scramble (NC) and CCDC132-shRNA (KD)-expressing lentiviruses were also simultaneously expressing green fluorescent protein (GFP); these cells were seeded and then captured the fluorescent signal every day; (B) and (D) then the cell number was autocalculated by manipulating those fluorescent signals in Celigo^® Image Cytometer and its software. (E) and (F) Colony formation analysis for the indicated cells. (G) Quantification of colony formation from (E) and (F). (H) FACS-based apoptosis analysis for the indicated cells. (I) Quantification of apoptosis ratio from (H). Results are presented as the mean ± SD. **p < 0.01; ***p < 0.001.