Fig. 5.
mRNA expression analyses of target genes in BMDM derived from wt (AhR+/+) or AhR null mice (AhR−/−). BMDM were treated with CARB ULSD (10 μg/ml), S100 (10 μg/ml), and TCDD (1 nM) used as positive control. Increased mRNA levels of A) CYP1A1 and B) COX-2 are shown and corrected against the housekeeping gene β-actin. Total RNA was prepared 24 h after treatment of BMDM derived from wt (AhR+/+) or AhR null mice ( AhR−/−). *, Values are the mean ± SD of three independent experiments and are significantly different from control (P < 0.005). Ctrl, Control.