Extended Data Table 1. Cryo-EM data collection, refinement and validation statistics.
| FA core complex (EMD-10290: consensus, EMD-10291: top, EMD-10292: middle, EMD-10293: base) (PDB 6SRI) |
Subcomplex (EMD-10294) (PDB 6SRS) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 75,000 X | 75,000 X |
| Voltage (keV) | 300 | 300 |
| Electron exposure (e−/Å2) | 40 | 40 |
| Defocus range (μm) | -1.8 to -4.0 | -1.8 to -4.0 |
| Pixel size (Å) | 1.040 (LMB) | 1.040 (LMB) |
| 1.085 (eBIC) | 1.085 (eBIC) | |
| Symmetry imposed | CI | CI |
| Initial particle images (no.) | 1,947,765 | 1,947,765 |
| Final particle images (no.) | 169,000 | 49,423 |
| Map resolution (Å) | 4.2 (consensus), 4.5 (top), 4.4 (middle), 4.9 (base) |
4.6 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 4.2 to > 10 | 4.6 to > 10 |
| Refinement | ||
| Initial model used | De novo modelling and homology modelling | De novo modelling and homology modelling |
| Model resolution range (Å) | 4.5 (top), 4.4 (middle), 4.9 (base) |
4.6 |
| FSC threshold | 0.143 | 0.143 |
| Model resolution range (Å) | n/a | n/a |
| Map sharpening B factor (Å2) | -149 (consensus) | -213 |
| -198 (top) | ||
| -190 (middle) | ||
| -177 (bottom) | ||
| Model composition | ||
| Non-hydrogen atoms | 15,309 | 12,424 |
| Protein residues | 3,827 | 3,106 |
| Ligands | 0 | 0 |
| B factors (Å2) | ||
| Protein | not estimated | not estimated |
| Ligands | ||
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.23 | 0.22 |
| Bond angles (°) | 0.48 | 0.50 |
| Validation | ||
| MolProbity score | 1.44 | 1.12 |
| Clashscore | 3 | 3 |
| Poor rotamers (%) | 0 | 0 |
| Ramachandran plot | ||
| Favored (%) | 95.95 | 98.88 |
| Allowed (%) | 3.56 | 1.12 |
| Disallowed (%) | 0.49 | 0 |