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. Author manuscript; available in PMC: 2020 Apr 7.
Published in final edited form as: Nat Methods. 2019 Oct 7;16(11):1146–1152. doi: 10.1038/s41592-019-0580-y

Figure 6. Sub-tomogram averaging results obtained by using Warp’s tilt series CTF estimation and sub-tomogram export.

Figure 6

To assess the benefits of the proposed CTF estimation and sub-tomogram export strategies, data from EMPIAR-10045 and EMPIAR-10164 were pre-processed and exported in Warp, and refined in RELION 3.0. Improved resolution was observed in both cases compared to published results.

(a) Isosurface rendering (left) and FSC plot (right) of the 80S ribosome sub-tomogram average obtained from EMPIAR-10045 data. The originally published resolution was 12.8 Å.

(b) Isosurface rendering (left) and FSC plot (right) of the HIV-1 sub-tomogram average obtained from 12% of the EMPIAR-10164 data. The originally published resolution for this subset was 3.9 Å.