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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Nat Struct Mol Biol. 2019 Nov 6;26(11):1053–1062. doi: 10.1038/s41594-019-0324-9

Figure 1. IRE1 and PERK LD cause loss of BiP ATPase stimulation by cochaperone and NEF effectors, but BiP retains its inherent activity.

Figure 1

a, Schematic representation of assay used for measuring ATPase activity. b-f, Graphs displaying phosphate (Pi) release over time as a result of BiP ATPase activity. Data shown are mean ± sd, from nine independent experiments. Source data available online. BiP displayed low basal ATPase activity; the addition of Sil1 slightly increased activity, whereas ERdj3 or both cochaperone Sil1 and ERdj3 together greatly stimulated BiP activity (b). Addition of IRE1 LD or PERK LD did not affect BiP basal ATPase activity (c) but reversed the stimulation by Sil1 (d), by ERdj3 (e), and by both cochaperones ERdj3 and Sil1 together (f). g, Bar graph showing the rates of BiP ATPase activity upon addition of effector proteins. Data are mean ± sd, from nine independent experiments. ATPase rates are in Supplementary Table 1.