Figure 4.

Physiological parameters during pure VILI model following terbutaline treatment. Initial elastance immediately after instillation and setting high V_T was no different between saline and terbutaline-instilled animals (A). Panel B shows overall elastance change and Panel C shows final arterial oxygen levels in animals following ventilation with 35ml/kg tidal volume (V_T 35). As animals develop injury at different rates, time courses of elastance (D) and oxygenation (E) were also evaluated. Note that ‘end’ relates to the final value, either after 3 hours of ventilation or whenever animals met the mortality surrogate. Panel F shows protein concentration in lavage fluid taken at the end of experiments. N=6/group for all datasets. End-point data in panels A, B & F were normally distributed, analysed by t-test and displayed as mean±SD. Data in Panel C required transformation before analysis and are displayed as back-transformed mean with 95% confidence intervals. *p<0.05, **p<0.01 vs vehicle (saline) by Students t-test. Time-course data in panel D were normally distributed and displayed as mean±SD while data in panel E required transformation before analysis and are displayed as back-transformed mean with 95% confidence intervals. Time courses were analysed by 2-way ANOVA. ***p<0.001, ****p<0.0001 for interaction. Exact p-values were as follows: Panel A p=0.1432; Panel B p=0.0030; Panel C p=0.0064; Panel D p<0.0001 (interaction); Panel E p=0.0004 (interaction); Panel F p=0.0041.