Physiological parameters following TNF receptor-1 domain antibody (dAb) treatment in two-hit LPS+VILI model. Initial elastance immediately after instillation and setting moderate VT was no different between groups (A). Overall elastance change (B) and final oxygenation (C) showed trends towards improvement with dAb treatment (P<0.1) in mice ventilated with 20ml/kg VT post-LPS. Time courses of elastance change (D) and oxygenation (E) were both significantly attenuated, as was final lavage fluid protein (F). N=6-7/group for all datasets. Data were all normally distributed and displayed as mean±SD, except for panel C which were non-normally distributed and displayed as box-whisker plot. End-point data were analysed by Students t-test (A, B, F) or Mann-Whitney U-test (C). *p<0.05 vs vehicle (dummy). Time course data (D, E) were analysed by 2-way ANOVA. *p<0.05, **p<0.01 for interaction. Exact p-values were as follows: Panel A p=0.4858; Panel B p=0.0712; Panel C p=0.0906; Panel D p=0.0089 (interaction); Panel E p=0.0399 (interaction); Panel F p=0.0295.