Table 3. Synthetic oligonucleotides for amplification of the CSBV genome.
Primers | Sequence (5′ to 3′) | Nucleotide position |
---|---|---|
S1 F | GAAATAAGAATACGAATCGT | 1–20 |
S1 R | TAAACAAATCGGTATAAGAGTCC | 379–401 |
S2 F | GACCCGTTTTCTTGTGAGTTTTAG | 41–64 |
S2 R | GTGTAGCGTCCCCCTGAATAGAT | 611–633 |
S3 F | CGTAATTGCGGAGTGGAAAGAT | 273–294 |
S3 R | CCCCTAAATTGTTGCGTTGGTT | 739–760 |
S4 F | TATTCAGGGGGACGCTACAC | 614–633 |
S4 R | TATTCCATCGGGGTTATTTG | 1,713–1,732 |
S5 F | GGAGACGCGCATGGTAAAGA | 1,644–1,663 |
S5 R | GCGCGGTAAATAAACACTCG | 2,365–2,384 |
S6 F | ATGGGGGTAAGGGACAATCTG | 2,290–2,310 |
S6 R | TGCTCTAACCTCGCATCAAC | 3,423–3,442 |
S7 F | TTACGGGAGCAGCACAACA | 3,391–3,409 |
S7 R | ATTTCCGATTTACCGATACC | 4,287–4,306 |
S8 F | CGGTGCGTTATGAACCTTTT | 4,243–4,262 |
S8 R | AATGCGTAGATTGAGGTGCC | 5,333–5,352 |
S9 F | TACTATCCGCCCCCTAAGC | 3,195–3,213 |
S9 R | GTGCCCCATCGTTCAAAA | 5,432–5,449 |
S10 F | GCGCAACTGGCACCTCAAT | 5,325–5,343 |
S10 R | TTCCAAATATACTTCCCACTGC | 6,249–6,270 |
S11 F | GCTGGGCCTTCTTATCTGGTG | 3,873–3,893 |
S11 R | TACGGGTCCTCTGCAATGTTCT | 6,363–6,384 |
S12 F | GTGACGGCAGTGGGAAGTAT | 6,262–6,243 |
S12 R | GCAGCCTCCTCAGGTGTTAGT | 7,454–7,474 |
S13 F | TTTGGTAGCGGGGTGTAAG | 7,322–7,340 |
S13 R | CATTGCGTGGTATCATT | 8,501–8,517 |