Skip to main content
. 2019 Nov 14;7:e8003. doi: 10.7717/peerj.8003

Table 3. Synthetic oligonucleotides for amplification of the CSBV genome.

Primers Sequence (5′ to 3′) Nucleotide position
S1 F GAAATAAGAATACGAATCGT 1–20
S1 R TAAACAAATCGGTATAAGAGTCC 379–401
S2 F GACCCGTTTTCTTGTGAGTTTTAG 41–64
S2 R GTGTAGCGTCCCCCTGAATAGAT 611–633
S3 F CGTAATTGCGGAGTGGAAAGAT 273–294
S3 R CCCCTAAATTGTTGCGTTGGTT 739–760
S4 F TATTCAGGGGGACGCTACAC 614–633
S4 R TATTCCATCGGGGTTATTTG 1,713–1,732
S5 F GGAGACGCGCATGGTAAAGA 1,644–1,663
S5 R GCGCGGTAAATAAACACTCG 2,365–2,384
S6 F ATGGGGGTAAGGGACAATCTG 2,290–2,310
S6 R TGCTCTAACCTCGCATCAAC 3,423–3,442
S7 F TTACGGGAGCAGCACAACA 3,391–3,409
S7 R ATTTCCGATTTACCGATACC 4,287–4,306
S8 F CGGTGCGTTATGAACCTTTT 4,243–4,262
S8 R AATGCGTAGATTGAGGTGCC 5,333–5,352
S9 F TACTATCCGCCCCCTAAGC 3,195–3,213
S9 R GTGCCCCATCGTTCAAAA 5,432–5,449
S10 F GCGCAACTGGCACCTCAAT 5,325–5,343
S10 R TTCCAAATATACTTCCCACTGC 6,249–6,270
S11 F GCTGGGCCTTCTTATCTGGTG 3,873–3,893
S11 R TACGGGTCCTCTGCAATGTTCT 6,363–6,384
S12 F GTGACGGCAGTGGGAAGTAT 6,262–6,243
S12 R GCAGCCTCCTCAGGTGTTAGT 7,454–7,474
S13 F TTTGGTAGCGGGGTGTAAG 7,322–7,340
S13 R CATTGCGTGGTATCATT 8,501–8,517