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. Author manuscript; available in PMC: 2019 Nov 17.
Published in final edited form as: Biochem Pharmacol. 2017 Dec 5;148:13–26. doi: 10.1016/j.bcp.2017.11.022

Fig. 5.

Fig. 5.

Downregulation of Mcl-1 and upregulation of Bim play an important role in cell death induced by the combination of VS-5584 and SCH772984 in AML cells. (A, B) MOLM-13 cells were treated with VS-5584 (VS), SCH772984 (SCH), or VS + SCH for 48 h (panel A) or up to 24 h (panel B). Normalized densitometry measurements for the Western blots are shown. # indicates p < .05 compared to control (n = 3, data not shown). (C) THP-1, OCI-AML3, and U937 cells were treated with VS, SCH, or VS + SCH for 12 h. Representative Western blots are shown with the corresponding normalized densitometry measurements below. * indicates p < .05 compared to VS alone (n = 3, data not shown). (D, E) U937 NTC or Bim shRNA knockdown (panel D) or Mcl-1 or RFP overexpression (panel E) was confirmed by Western blotting. Normalized densitometry measurements are indicated. ** indicates p < .01 compared to NTC (n = 3), while # indicates p < .05 compared to RFP (n = 3). U937 NTC and Bim shRNA or U937 RFP and Mcl-1 cells were treated with VS, SCH, or VS + SCH for 12 h or 48 h and then subjected to Annexin V/PI staining and flow cytometry analysis. *** indicates p < .001.