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. Author manuscript; available in PMC: 2020 Apr 7.
Published in final edited form as: Nat Immunol. 2019 Oct 7;20(11):1530–1541. doi: 10.1038/s41590-019-0489-8

Fig. 8. ARGHAP45 is crucial for proper T and B cell migration.

Fig. 8

a, Quantification of CD45+ cells in the blood of wild-type mice and of mice with biallelic deletions of the Arap1, AI467606, Arhgap45, Cep85l, and Nap1l4 genes. Each symbol corresponds to a mouse and the mean (horizontal bar) is indicated. Among the analyzed Arghap45 F0 mice, six contain one Arghap45 allele with a small deletion preserving its open reading frame and were used as internal control giving rise to normal blood phenotype. Wild-type: n = 25; Arhgap45: n = 56; Arap1, n=16; C16orf54: n = 27; Cep85l: n= 27; Nap1l4: n = 33. For each mutation, comparison was done relative to wild-type (WT) mice using unpaired Mann–Whitney’s t-test. ns, nonsignificant. *** P = 0.0002; **** P = 0.000004. In a to d, only significant values (P< 0.05) were specified. b, Quantification of CD8+ T cells, CD4+ T cells, B cells, NK cells, neutrophils, monocytes and eosinophils in the blood of wild-type mice (n = 14) and of F0 mice with biallelic deletions in the Arghap45 gene (n = 32). *** P = 0,00021; ****, P < 0.00001 (unpaired Holm-Sidak’s multiple t test corrected for multiple comparisons). c, Cellularity of the thymus, spleen, peripheral (p) and mesenteric (m) lymph nodes of the progeny of wild-type mice (n = 20) and of F0 mice with biallelic deletions in the Arghap45 gene (n = 20). ****, P< 0.00001 (unpaired Holm-Sidak’s multiple t test corrected for multiple comparisons). d, Quantification of γδ T cells, CD8+ T cells, conventional CD4+ T cells, regulatory CD4+ T cells, NK cells, and follicular B cells in the mesenteric lymph nodes of wild-type mice (n = 16) and of F0 mice with biallelic deletions in the Arghap45 gene (n = 16). *, P = 0.017; **, P = 0.004; ***, P = 0.00003; *****, P < 0.00001 (unpaired Holm-Sidak’s multiple t test corrected for multiple comparisons).