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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Nature. 2019 Oct 2;574(7777):278–282. doi: 10.1038/s41586-019-1609-1

Figure 2. Structure of the S. cerevisiae CCAN–Cenp-ANuc complex.

Figure 2

a, Cryo-EM density map of CCAN–Cenp-ANuc. Cenp-AN: residues 111-129. b, c, Two views of a cartoon representation of CCAN–Cenp-ANuc. Cenp-ANuc wraps ~105 bp of DNA, leaving 20 bp of DNA unwrapped at both ends (coloured yellow for the ordered terminal segment). Supplementary Video 1. d-f, Three views of the cryo-EM density of a 3D sub-class of the overall CCAN–Cenp-ANuc 3D class, before application of the mask used to refine the cryo-EM map shown in (a) (Extended Data Fig. 3a), highlighting contacts to Cenp-ANuc. d, The Cenp-HIKHead module contacts Cenp-A. e, Cenp-THFDW contacts the DNA gyre of Cenp-ANuc. f, N-terminal region of Cenp-QU contacts Cenp-A and H4.