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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Nature. 2019 Oct 2;574(7777):278–282. doi: 10.1038/s41586-019-1609-1

Figure 3. Cenp-LN interacts with the unwrapped DNA duplex of Cenp-ANuc.

Figure 3

a, b. Two orthogonal views showing the unwrapped DNA duplex of Cenp-ANuc engaged by the DNA-binding groove of the Cenp-LN sub-complex. c, Surface of Cenp-LN showing positive electrostatic potential of the DNA-binding groove. The canonical S. cerevisiae H3 nucleosome (orange, PDB: 1ID3 24) wraps 147 bp of DNA compared with the 105 bp wrapped by the S. cerevisiae Cenp-A nucleosome (yellow). d, Zoomed-view showing insertion of the N-terminus of Cenp-A (Cenp-AN) between the unwrapped DNA duplex and DNA gyre of Cenp-ANuc. Arg67 of the Cenp-N Pyrin domain inserts into the DNA major groove.