Supplementary Table 3. List of rare variants in ACTC1, MYBPC3, MYH6 and MYH7 genes.

Patient ID	Gene	Mutation type	AA change	Exon	Domain	Nucleotide change	dbSNP	ClinVar	AMA and ACMG recommended guidelines	SIFT	Polyphen2	Mutation taster	1000 Genomes ASN	ExAC ASN	Normal control (allele fre, %)
NGS056	ACTC1	Missense	p.A274P	6	Actin	c.G820C	-	-	Uncertain significance	D	D	D	0	0	0
NGS024	MYBPC3	Missense	p.R238H	6	IgC-1	c.G713A	rs727504396	Uncertain significance	Uncertain significance	D	D	D	0	0	0.0004
NGS018	MYBPC3	Nonsense	p.Q508X	16	IgC-3	c.C1522T	rs730880544	Pathogenic	Likely pathogenic	T	-	A	0	0	0
NGS070	MYBPC3	Missense	p.G835E	24	IgC-3	c.2504_2505TT	-	-	Uncertain significance	-	-	D	0	0	0
NGS028	MYBPC3	Missense	p.R470Q	15	IgC-3	c.G1409A	rs776734314	Uncertain significance	Uncertain significance	D	D	D	0	0	0
NGS018	MYBPC3	Missense	p.P186L	5	IgC-1	c.C557T	rs727503216	Uncertain significance	Uncertain significance	D	P	D	0	0.0003	0
NGS021	MYH6	Nonsense	p.R1923X	38	Myosin tail	c.C5767T	rs765861895	Uncertain significance	Likely pathogenic	T	-	A	0	0	0
NGS015	MYH7	Missense	p.P527S	16	Myosin motor	c.C1579T	rs1437377039	-	Uncertain significance	D	P	D	0	0	0

* Coding reference used in this table: ACTC1, NM_005159; MYBPC3, NM_000256; MYH6, NM_002471; MYH7, NM_000257.

A, disease causing automatic, D, Deleterious/Disease causing; P, probably damaging; T, tolerated.