Figure 2. miR-365 down-regulates IGF-I by directly targeting its 3′UTR.

(A) miR-365 target sequence alignment in the IGF-I 3′UTR. (B) Activity of a luciferase reporter fused to IGF-I 3′UTR and IGF-I 3′UTR mutated fragments transfected into duck myoblast that were kept in growing DMEM. (C) Influences of miR-365 mimic overexpression on IGF-I expression. (D) Influences of anti-miR-365 overexpression on IGF-I expression. Each point represents the relative mean ± SD. * denotes significance (P<0.05).