Table 1. Primer sequences used in the present study.
| Primer ID | Primer name | Primer sequence (5′–3′) |
|---|---|---|
| P1 | Psi-IGF-I-F | CCGCTCGAGACCTGAGGAGGCTGGAGATGTACTG |
| Psi-IGF-I-R | ATAAGAATGCGGCCGCGAATGTTTAGTTGCATTGTTCACTGGG | |
| P2 | Psi-IGF-I-MUT-F | AACCAATTTTACGGCTCCCAGT GAACAATGCAACTAAACATTCCAATATT |
| Psi-IGF-I-MUT-R | ACTGGGAGCCGTAAAATTGGTTGAGATTGCATCAAGCTTTTTAACCAT | |
| P3 | Psi-CCND1-F | CCGCTCGAGTTCTTGCTCTGTCTCCCTTCCATCT |
| Psi-CCND1-R | ATAAGAATGCGGCCGCAGCCTAGAACTGCGTTAAAGCTATGCT | |
| P4 | IGF-I-F | GTGAAGATGCATACTGTGTC |
| IGF-I-R | TGAAGTAAAAGCCTCTGT | |
| P5 | PI3K-F | CTTTTACCGAGGAGGTTCTGTGG |
| PI3K-R | CTGAAGGTTGGTCTTTGTGGAC | |
| P6 | Akt-F | TCTTTGCTGGCATTGTTTGGC |
| Akt-R | GCTGTCATCTTGGTCAGGAGGAGT | |
| P7 | mTOR-F | CTATCTGCCTCAGCTCATTCCT |
| mTOR-R | GTCATCCAGGTTAGCTCCAAAG | |
| P8 | p70S6K-F | ATAATCGTGCTGTGGACTGGTG |
| p70S6K-R | TCTGGCTTCTTGTGTGAGGTAGG | |
| P9 | p21-F | TTGGCTCACAAGGTCCCATCTAAGG |
| p21-R | TTGGCTCACAAGGTCCCATCTAAGG | |
| P10 | CCND1-F | CTGGATGCCAACCTCCTCAACG |
| CCND1-R | GCACTTCTGCTCCTCGCAAACCT | |
| P11 | CCND2-F | ACAATCCCCCTCACAGCAGAGAAGC |
| CCND2-R | TGCGAGGTTCCACTTCAACTTCCC | |
| P12 | CCND3-F | ACAGCACGAGGCGAGCAAGGAG |
| CCND3-R | GGGACCGAGGACAGGTAGCGAT | |
| P13 | GADPH-F | AAGGCTGAGAATGGGAAAC |
| GADPH-R | TTCAGGGACTTGTCATACTTC |
F,R: forward and reverse primers, respectively. Ta Opt means annealing temperature.