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. 2019 Nov 13;12:9585–9598. doi: 10.2147/OTT.S215674

Figure 3.

Figure 3

High coronin3 expression was correlated with stronger migration and invasive properties in NPC cell lines. (A) Coronin3 mRNA overexpressed in CNE-2 compared with CNE-1 according to GSE15047. (B) The mRNA expression of coronin3 was estimated by qPCR. β-actin was used as the internal control. 2-ΔΔCT method was used for results analysis. ***p<0.001. n=3 per group, three repeats per time. (C) The protein expression of coronin3 was estimated by Western Blotting. (D) The migratory and invasive ability was evaluated by counting the number of cells that transferred the 8-μm-pore transwell membrane with or without the Matrigel. *p<0.05; ***p<0.001. Magnification ×200, n=3 per group. (E) Wound-healing assay was performed by assessing the closure at 0 hr, 24 hrs and 48 hrs. **p<0.01; ***p<0. 001. Magnification ×200, n=3 per group.