Fig. 1.

Effects of RPG-OM-30E on NO production in LPS-stimulated RAW 264.7 cells. (A) Scheme for fraction of RPG-OM-30E from Omija byproduct. (B) LPS-stimulated RAW 264.7 cells were used for testing the inhibition of NO production. Inhibitory effects of fermented and unfermented omija hot water extract on NO production were compared in the concentration of 5 and 10%. (C) The inhibition of NO production by ethanol fractions of fermented omija hot water extract was tested at the concentration of 0.1 mg/mL. (D) Cell viability was determined through the WST assay. Cells were treated with each concentration of RPG-OM-30E for 24 h. Values are expressed as percentages of the control (0.1% DMSO). (E) The effects of RPG-OM-30E on LPS-induced NO production were measured using the Griess reagent. Cells were stimulated with 1 μg/mL of LPS in the presence of each concentration of RPG-OM-30E. NO production was measured after 24 h. Significant compared to the group treated with LPS alone, *p < 0.05, **p < 0.01, and ***p < 0.001