Fig. 4.
Inhibitory effects of RPG-OM-30E on neutrophil migration toward the wound site. A neutrophil migration assay was carried out using Tg(mpx::EGFP)i114 larvae. (A) Fluorescent neutrophils were observed at the tailfin of zebrafish larvae (white arrows). Control and positive control larvae were treated with 0.1% DMSO and 100 μM dexamethasone, respectively. RPG-OM-30E was applied at concentrations of 0.1 and 0.5 mg/mL. (B) Quantification of neutrophils recruited to the injury site (n > 15). The number of neutrophils was counted at the tailfin of zebrafish larvae. The data represent the mean ± SD of three independent experiments. Significant differences compared to the control group, **p < 0.01