Fig. 1.

Schematic representation of the computational process used for reconstruction and comparison of the PPI network of MRU with gut methanogens. 1. Retrieval of annotated proteome information of MRU genome, 2. Retrieval of annotated proteome information of existing genome-scale models, 3. Construction of PPI network and enrichment of molecular and biological function of proteins, 4. Computational evaluation of network properties of each PPI network, 5. Network validation and functional annotation of metabolic enzymes, and 6. Comparison of differential networks of MRU with heterotrophic and hydrogenotrophic methanogens.