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. 2019 Nov 15;2(6):e201900462. doi: 10.26508/lsa.201900462

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© 2019 Simões et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — (A) Time course levels of endogenous expression of TMEM16A protein during differentiation of BCi-NS1.1 cells grown at ALI (days 1–30) detected by WB, showing both its non-glycosylated (∼100 kD) and glycosylated forms (∼120 kD). GAPDH (∼36 kD) was used as a loading control. (A, B) Quantification by densitometry of TMEM16A total protein levels from (A) normalized to the loading control shown as mean ± SEM (n = 3). (C) Fold-change in TMEM16A mRNA expression levels as a time course of differentiation of BCi-NS1.1 cells grown at ALI (days 0, 15, and 30), determined by qRT-PCR. Fold-change values are mean ± SEM, relative to the mean value of day 30 (n = 3). (D) Time course levels of endogenous expression of MUC5AC (>300 kD) analysed by WB during differentiation of BCi-NS1.1 cells. Vinculin was used as a loading control (∼120 kD). Dashed line indicates lanes run on the same gel but noncontiguous. (D, E) Quantification by densitometry of MUC5AC protein levels from (D) normalized to the loading control shown as mean ± SEM (n = 4). (F) Fold-change in MUC5AC mRNA expression levels as a time course of differentiation of BCi-NS1.1 cells grown at ALI (days 0, 15, and 30), determined by qRT-PCR. Fold-change values are mean ± SEM, relative to the mean value of day 30 (n = 4). (G) Correlation of TMEM16A and MUC5AC normalized protein levels during differentiation and TEER measurements. Asterisks and cardinals indicate significant difference compared with day 0 (P-value < 0.05, unpaired t test).

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