Fig. 4.

Maximum likelihood phylogeny of VasX homologs and their gene clusters. Sixteen representative VasX homologs were aligned by MUSCLE and phylogeny (bootstrap = 100) was generated by PhyML using the MPI Bioinformatics Toolkit with default settings. Bootstrap value for each branch is indicated. E. coli colicin S4 protein, the structural template used for VasX modeling, serves as an outgroup. Gene clusters are retrieved from National Center for Biotechnology Information and curated. The size of each gene is not to scale.