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. 2019 Sep 30;116(46):23254–23263. doi: 10.1073/pnas.1907490116

Fig. 2.

Fig. 2.

HMGB1 and C1q reciprocally regulate phosphorylation and nuclear localization of 5-LO. (A) Nuclear localization of 5-LO induced by HMGB1 and/or C1q stimulation for 1 h; nucleus marked by PI (blue) and 5-LO stained with rabbit anti–5-LO and Alexa Fluor 488 anti-rabbit IgG (red). The ratio of nuclear/nonnuclear 5-LO was determined in more than 80 cells in each condition (mean ± SEM; n = 4). (Scale bar, 10 µm.) *P < 0.05 and **P < 0.01 (1-way ANOVA). (B) Immunoblot for serine-271 phosphorylation of 5-LO in total cell lysates from HMGB1 or HMGB1-plus-C1q–stimulated monocytes (1 h). The fold induction of phospho-5-LO in HMGB1 and/or C1q-stimulated monocytes is compared to untreated samples from 3 independent experiments. *P < 0.05 and **P < 0.01 (1-way ANOVA).