Fig. 2.

ScSERF has minimal effect on self-seeded amyloid kinetics. (A) Normalized ThT fluorescence of 25 μM Aβ40 self-seeded with 0.5 μM preformed Aβ40 fibrils (2% seeds) incubated in the absence (blue) or presence of different concentrations of ScSERF. (B) Same as in A, but self-seeded with 2.5 μM preformed Aβ40 fibrils (10% seeds). (C) Self-seeding assay with 100 μM α-synuclein and 5% seeds. (D) Self-seeding assay with 100 μM α-synuclein and 10% seeds. In all experiments, assays were conducted under quiescent conditions at 37 °C (20 mM NaPI, 50 mM NaCl, 200 μM EDTA, pH 7.4, and 25 μM ThT).