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. 2019 Oct 28;116(46):23152–23162. doi: 10.1073/pnas.1910960116

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Fig. 6. — Rescue of drc4 (pf2) and drc5 (sup-pf4) mutants by transformation with SNAP-tagged DRC subunits. (A) The DRC4 and DRC5 protein sequences are drawn to scale, with the location of predicted polypeptide domains indicated: CC, coiled-coil domain; LRR, leucine-rich repeat; SNAP, SNAP tags introduced near the N terminus of DRC4 and the C terminus of DRC5. (B) The forward swimming velocities of drc mutants as measured by phase contrast microscopy are shown relative to those of wild-type (WT) and rescued strains generated by transformation with the indicated SNAP-tagged DRC gene. As indicated by the asterisks (*P < 0.05; ***P < 0.001), both drc mutants were significantly slower than WT and the SNAP-tagged rescue strains, but the latter were also slightly slower than WT. (C and D) Western blots of axonemes isolated from WT, drc mutants, and SNAP-tagged rescued strains were probed with antibodies against several DRC subunits. Note the presence of bands that migrated at the sizes predicted for SNAP-tagged DRC subunits. An antibody against the IC2 subunit of the outer dynein arms served as a loading control.