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. 2019 Oct 28;116(46):23124–23131. doi: 10.1073/pnas.1904813116

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Fig. 1. — A FRET biosensor for measuring effective concentrations. (A) Titration with free MBD2 peptide displaces the intramolecular interaction and results in a decrease in the FRET efficiency. (B) Structure of the interaction pair in the fusion protein: the antiparallel coiled coil formed between MBD2 and p66α (PDB ID code 2L2L) (39). (C) SDS/PAGE gel of the purified fusion protein reveals internal cleavage sites in mRuby3. The cleavage products cannot be removed, but do not affect measurements.