Fig. 3.

Net charge per residue dominates the scaling of effective concentration with linker length. (A) Net charge was uniformly distributed into a background of GS-linkers. The same pattern is used for subsequent linker series. (B) Measurement of effective concentration allows determination of scaling exponents for each linker composition. Error bars represent 95% CI. (C) Scaling exponents demonstrate a linker expansion with increasing net charge. Compared to the negatively charged residues, arginine expands the chain less and lysine more. Error bars represent SE.