Fig. 5.

Lack of additivity of individual contributions. (A) Proline or leucine residues were introduced in equal proportion to glutamate residues, but the scaling coefficient follows that of the glutamate-only series. This suggests that the expansion caused by net charge and chain stiffness are not additive. (B) Charge expansion caused by glutamate against a constant fraction of 0.1 of leucine residues shows that hydrophobicity has a negligible effect. (C) Increasing fractions of leucine only has a negligible effect on the dimensions of the linkers expanded by charge. Error bars represent SE.