Fig. 1.

Mouse adipose Pm20d1 is induced by cold but not PPARγ agonist drug. (A) Wild-type mice were housed at room temperature versus 4 °C for 24 h (acute cold) or 7 d (chronic cold, n = 5 per group), and gene expression measured by qPCR in inguinal WAT. (B) Wild-type mice were treated with rosiglitazone (rosi) versus control diet (n = 4 per group), and gene expression measured by RNA-seq in epididymal WAT. (C) Mouse 3T3-L1 adipocytes were treated for 24 h with 1 μM rosiglitazone versus vehicle (DMSO, n = 6 wells each). Mean and SEM, ^#P < 0.001; *P < 0.01; ^P < 0.05; NS, not significant by 2-tailed type 2 Student’s t-test.