Skip to main content
. 2019 Oct 28;116(46):23232–23242. doi: 10.1073/pnas.1913199116

Fig. 2.

Fig. 2.

PM20D1 expression is highly induced by PPARγ agonist in human adipocytes. (A) SGBS cells were cultured in adipocyte differentiation media including rosiglitazone (rosi) for 8 d, then in maintenance media with or without rosi for 11 d. Rosiglitazone or vehicle was then reintroduced to mature adipocytes for 2 d. n = 6 wells per condition, and this schema is the color key for samples in B and C. (B and C) Expression of PM20D1 or PDK4 was assayed by qPCR and normalized to 36B4 expression, with levels in mature adipocytes set equal to 1. Mean and SEM, #P < 0.001 by Student’s t-test. (D) Browser track of published human adipocyte PPARγ ChIP-seq at the PM20D1 locus, from either SGBS or primary adipocytes from human adipose stem cells (hASC) (13–15).