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. 2019 Oct 23;203(11):3054–3067. doi: 10.4049/jimmunol.1900863

FIGURE 4.

FIGURE 4.

Accumulation of IgT+ B cells in the pharynx of trout infected with I. multifiliis. (A–C) Representative differential interference contrast (DIC) images of immunofluorescence staining on paraffinic sections of pharynx from uninfected control fish (A), 28 d–infected fish (B), and survivor fish (C). IgT+ and IgM+ B cells were stained with rabbit anti-trout IgT (green) and mouse anti-trout IgM (red), respectively; nuclei were stained with DAPI (blue) (isotype-matched control Ab staining, Supplemental Fig. 2B). (D) Enlarged images of the areas outlined in (C) are showing some IgT+ B cells possibly secreting IgT in pharyngeal epithelium (white arrowhead). Data are representative of at least three independent experiments (n = 9 per group). Scale bar, 20 μm. (E) Numbers of B cells in control, 28 d–infected, and survivor fish counted from (A)–(C). (F and G) Concentration of IgT, IgM, and IgD in pharyngeal mucus (F) and serum (G) from uninfected control fish, 28 d–infected fish, and survivor fish (n = 12). Data in (E)–(G) are representative of at least three independent experiments (mean ± SEM). *p < 0.05, ***p < 0.001 (one-way ANOVA with Bonferroni correction). PE, pharyngeal epithelium.