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. 2019 Oct 23;203(11):3054–3067. doi: 10.4049/jimmunol.1900863

FIGURE 7.

FIGURE 7.

Local IgT-, IgM-, and IgD-specific responses to I. multifiliis parasite of survivor fish. Pharynx, head kidney, and spleen explants (∼30 mg each) from control and survivor fish were cultured in medium (400 μl) for 7 d. (A–C) Western blot analysis of IgT-, IgM-, and IgD-specific binding to I. multifiliis in the culture medium of pharynx (A), head kidney (B), and spleen (C) (dilution 1:2) from control and survivor fish. (D–F) IgT-, IgM-, and IgD-specific binding to I. multifiliis in dilutions of pharynx (D), head kidney (E), and spleen (F) culture medium from survivor fish, evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12). Data are representative of at least three independent experiments (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student t test).