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Expression of PIK3CD alternate 5' UTR transcripts in the adult and fetal human brain. Isoform specific primers were used (Table 1) to amplify cDNA transcripts from fetal and adult brain. PCR confirms a predicted amplicon size of ~3.2 kb for each PIK3CD 5' UTR isoform. 5' UTR -2B was poorly expressed in fetal brain and undetectable in adult neocortex.
